Many of the members are former prostitutes who, rather than killed for attempting to escape, were hidden amidst the Hyakka's ranks by Tsukuyo.
While not having a uniform, all members wear similar kimonos with varying designs and obi. They also sport the same shimada hairstyle as any other courtesan in Yoshiwara. With some exceptions, the Hyakka have scars on their faces which are normally hidden behind masks.
The Hyakka, along with Tsukuyo first appear when they intercept Seita 晴太 and the Yorozuya 万事屋. They soon leave after Tsukuyo seemingly kills the intruders. They quickly discover their Leader's betrayal and intercept her and the Yorozuya as they try to reach Housen. They find themselves unable to kill Tsukuyo, who refuses to fight back, as many of them ower their lives to her. Later, some assist Gintoki in fighting Housen, to little result, while others help Hinowa 日輪 and Seita 晴太 escape. They are stopped by the Hyakka who are still loyal to the Night King 夜王.
Later, when Jiraia attemps to burn Yoshiwara down, the Hyakka and residents of the city prevent the fire from spreading.